Part:BBa_J23018

EN-Ptet-rbs-X-[RFP]-SNP "ORF Expresser"

Variant of pBca1021 (J61003) with GFP inserted. In practice, it is a E0040 basic part just in a different pSB1A* plasmid. Part J23019 is the same as J23018 but in pSB3C6.

• RS 16:08, 12 September 2008 (UTC): Note that this plasmid has been split into a plasmid backbone and part. The plasmid backbone is this part (BBa_J23018) and the part is BBa_E1010. The physical DNA associated with this part has sequence BBa_J23018-E1010.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Plasmid lacks a prefix.
  Illegal EcoRI site found at 2058
  Illegal XbaI site found at 2292
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 2058
  Illegal SpeI site found at 2
  Illegal PstI site found at 16
  Illegal NotI site found at 9
  Illegal NotI site found at 2064
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 2058
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Plasmid lacks a prefix.
  Illegal suffix found at 2
  Illegal EcoRI site found at 2058
  Illegal XbaI site found at 2292
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 2058
  Illegal XbaI site found at 2292
  Illegal SpeI site found at 2
  Illegal PstI site found at 16
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal BsaI.rc site found at 1097